Master GREEN 200 manuals

Master GREEN 200 User Manual (56 pages)

Brand: Master | Category: Power generators | Size: 0.46 MB |

English

Table of contents

Green PCR Master Mix

1

Contents

3

3 Reverse Transcription

4

5 Data Analysis

5

A References

6

B Technical Support

6

Introduction 1

7

Purpose of the Kit

8

Materials and Equipment

9

RISM™ 384-Well Clear Optical

10

RISM™ Optical Adhesive

10

Storage and

11

Stability

11

Documentation

12

User Attention

12

Chemical Hazard

12

Hazard Warning

13

Site Preparation

13

About MSDSs

13

Chemical Waste

13

Overview

15

Hot Start PCR

15

AmpliTaq Gold

15

DNA Polymerase

15

False Positives

16

Optional Use of

16

AmpErase UNG

16

Introduction 1-11

17

1-12 Introduction

18

Introduction

19

Dissociation Curve

19

Using Dissociation

19

Using Agarose

20

Gels to Check PCR

20

Product Purity

20

Identifying Target

22

Sequence and

22

Amplicon Size

22

Designing Primers

22

Selecting an

23

Amplicon Site for

23

Genomic DNA

23

Ordering Reagents

24

Quantitating

24

Reverse

25

Transcription 3

25

Guidelines

26

Two-Step RT-PCR

27

RT Reaction Mix

27

Performing RT

28

Reactions

28

Recommended

29

Template

29

Template Quality

29

Template Quantity

29

Preparing the

30

CAUTION!

31

(continued)

32

Thermal Cycling

33

Optimizing Primer

35

Concentrations 4

35

Concentrations for

36

PCR Master Mix

37

Optimization

37

Activation

38

One-Step RT-PCR

40

Master Mix for

40

Confirm the

42

Absence of

42

Nonspecific

42

Amplification

42

Data Analysis 5

47

5-2 Data Analysis

48

Interpreting the Results

49

References A

51

Technical Support B

53

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